Coding

Part:BBa_K3796003:Design

Designed by: Li Mingyang   Group: iGEM21_CAU_China   (2021-09-04)


capB from Bacillus subtilis str.168


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 737
    Illegal XbaI site found at 1032
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 737
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 737
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 737
    Illegal XbaI site found at 1032
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 737
    Illegal XbaI site found at 1032
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A segment of the RBS recognized by the ribosome of Corynebacterium glutamicum and a promoter recognized by the sigma factor needs to be attached upstream of this sequence, as well as a segment of the terminator downstream.


Source

Bacillus subtilis str.168 genome.

References